Siena, a UNESCO World Heritage Site and one of Tuscany’s most enchanting cities, is a perfect destination for a day trip from Florence. Trains run regularly from Florence's Santa Maria Novella Station and buses from Firenze Autostazione (Florence Bus Station). The journey takes about 1.5-2 hours. Many tour operators in Florence offer day trips to Siena, often combined with stops at other Tuscan destinations like San Gimignano or Chianti wineries.